Figure 8

SSR240612 improved evoked CBF responses to sensory stimulation in vivo and cerebrovascular reactivity ex vivo . (A) In APP mice, B₁R antagonism (+) significantly ameliorated, but did not fully restore, the cerebral blood flow (CBF) response evoked by whisker stimulation. (B,C) Isolated and pressurized middle cerebral artery segments from APP mice (▲) displayed impaired dilation to acetylcholine (ACh) (B) and reduced, albeit non-significantly, the biphasic response (contraction followed by relaxation) to bradykinin (BK) (C). Treatment with SSR240612 in APP mice (Δ) completely rescued dilation to ACh (B) but had no significant effect on vascular responses to BK (C). Error bars represent SEM. *P <0.05; **P <0.01, ***P <0.001, Two-way ANOVA followed by Newman-Keuls post hoc test. N = 4 to 6/group. (● WT; ○ WT treated). APP, amyloid precursor protein; B₁R, bradykinin receptor 1; WT, wild-type.