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Table 2 Effect of various signal transduction pathway inhibitors on [ 3 H]saquinavir accumulation in microglia

From: Microglial activation decreases retention of the protease inhibitor saquinavir: implications for HIV treatment

Inhibitor Concentration Saquinavir transport (% control)
   10 ng/ml LPSa Inhibitor alone LPS + Inhibitor
Anti-TLR2 1 μg/ml 62 ± 2 99 ± 3 b82 ± 2
Anti-TLR4 1 μg/ml 62 ± 2 99 ± 4 b75 ± 3
Fucoidan 50 ng/ml 67 ± 3 92 ± 5 57 ± 2
DPI 10 nM 63 ± 2 94 ± 9 64 ± 11
Tempol 20 μM 63 ± 2 88 ± 7 60 ± 3
IL-1β RA 10 μg/ml 73 ± 2 102 ± 4 77 ± 3
Anti-IL-1β 0.5 μg/ml 63 ± 3 91 ± 2 64 ± 2
Anti-TNF-α 0.5 μg/ml 63 ± 3 89 ± 2 64 ± 2
TIMP3 10 ng/ml 57 ± 4 97 ± 5 61 ± 2
1400 W 5 μM 61 ± 3 94 ± 3 70 ± 2
L-NMMA 10 μM 58 ± 3 98 ± 7 63 ± 2
BIM 25 nM 61 ± 4 92 ± 3 67 ± 6
Wortmannin 1 μM 70 ± 2 99 ± 6 68 ± 3
SB203580 10 μM 63 ± 2 93 ± 2 63 ± 3
JNKII 10 μM 66 ± 3 96 ± 2 64 ± 3
  1. Pathway inhibitor acronyms defined in text. a P < 0.05 as compared to untreated controls. b P < 0.05 as compared to LPS alone and untreated controls (partial inhibition).