Figure 6

Quantification of biotinylated dextran amine-positive corticospinal tract fibers in recombinant IL-1β-treated or IL-1βKO mice. (A) The quantity of corticospinal tract (CST) fibers (shown as a percentage of the total number of biotinylated dextran amine (BDA)-positive fibers at C4 level in a standardized, 20 μm wide area [18]) was significantly decreased 5 mm caudal to the lesion in recombinant IL-1β (rIL-1β)-treated mice compared with controls. (B) Conversely, the percentage of CST fibers of IL-1βKO mice increased about fivefold compared with controls. Bars represent the percentage of CST fibers at the lesion center (LC) and in the area 0.5 mm, 2 mm and 5 mm distal to the LC. *P <0.05; n = 7 mice (PBS), n = 6 mice (rIL-1β), n = 9 mice (C57BL6/J), n = 6 mice (IL-1βKO). Values throughout are represented as mean ± standard error of the mean and P values were determined using the Mann–Whitney U-test.