Figure 6

Analysis of dendritic cell, M1 and M2 markers displays a similar pattern between DA and Aplec rats. Global expressional profiling of spinal cords from naïve and operated DA and Aplec rats enabled a detailed study of CD11c, a marker commonly used for dendritic cells (DCs), as well as markers used to differentiate between M1 (pro-inflammatory) and M2 (anti-inflammatory) macrophages and microglia. CD11c expression was unaffected by strain or injury, arguing against large differences in DC activity (A). iNOS (inducible NO-synthase), CD16, CD86 and CD32 are typical M1 markers (B-E) whereas arginase 1, mannose receptor 1/CD206, CD163, CD14 and CD23 are typical M2 markers (F-J) and they all showed similar patterns without strain differences, where most were significantly upregulated in both DA and Aplec rats after injury. Aplec: antigen-presenting lectin-like receptor gene complex; DC: dendritic cell’ iNOS: inducible NO-synthase; MR1: mannose receptor 1.