Reduction in infarct size by EA treatment in wild-type but not in MCPIP1 knockout mice. The brain infarct size was assessed 48 h after MCAO. (A) Infarct images obtained by TTC staining at 48 h after MCAO. The normal tissue was stained deep red and the infarct was stained milky. (B) Brain infarcts were quantified as percentage area of ischemic hemisphere. The infarct size of EA-pretreated wild-type mice was significantly reduced compared to that of control. There was no significant difference in brain infarct size between EA-pretreated and control in MCPIP1 knockout mice. Values represent mean ± SD, *P <0.05, n = 10 mice per group. EA, electroacupuncture; MCAO, middle cerebral artery occlusion; MCPIP1, monocyte chemotactic protein-induced protein 1; SD, standard deviation; TTC, 2,3,5-triphenyltetrazolium chloride.