Figure 4
From: Neurogenic factor-induced Langerhans cell activation in diabetic mice with mechanical allodynia
Increased aggregation of CD68-positive cells in the subepidermal plexus of db/db mice. Representative images of PGP 9.5/CD68 double immunohistochemistry of hind foot pads from db/+ (A,B,C) and db/db (D,E,F) mice at 8 weeks of age. PGP 9.5 immunohistochemistry demonstrates IENFs (A,D, arrowheads,) and nerve fibers located in the subepidermal nerve plexus (A,C,D,F, asterisks). Increased quantities of subepidermal CD68-positive cells (B,C,E,F, arrows) were detected in the subepidermal plexus (A,C,D,F, asterisks). Bar = 50 μm, n = 8. Epi, epidermis; IENF, intraepidermal nerve fiber; PGP, protein gene product.