Figure 7
From: Neurogenic factor-induced Langerhans cell activation in diabetic mice with mechanical allodynia
Intrathecal SB203580 treatment reduces TNF-α expression and the quantity of CD68-positive cells in the subepidermal plexus of db/db mice. Representative images of TNF-α/CD68 double immunohistochemistry of hind foot pads from db/+ (A,B) and db/db (C,D) mice at 8 weeks of age after 1 week of intrathecal artificial cerebrospinal fluid (CSF) or SB203580 (SB) treatment. Compared to the CSF-treated db/db mice, SB203580 treatment reduced the expression of TNF-α in the subepidermal plexus (compare C and D, arrowheads) in the db/db treated mice. In contrast, SB203580 treatment did not affect TNF-α (arrowheads) or CD68 (arrows) expression in the subepidermal plexus of db/+ mice (compare A and B). Bar = 50 μm, n = 8. CSF, cerebrospinal fluid; Epi, epidermis.