Figure 5

CD95 expression in the Grid2 ^Lc/+ cerebellum. (A) Western blot analysis of the CD95 protein in crude extracts of cerebral cortex and cerebellum from Grid2^Lc/+(Lc) mutant and wild-type (wt) mice. Thymus extracts from lpr and wt mice were used as negative and positive controls, respectively. (B) CD95 western blot analysis at two time points - postnatal day 22 (P22) and P52 - in wt and Lc cerebella, and relative densitometric quantification of the 52 kDa band, expressed as a percentage of a reference protein sample. Values are the means ± SEM of four independent determinations (one-way analysis of variance followed by de Sheffé multiple comparison test, *P <0.05, **P <0.01). (C) Time course of CD95 mRNA production in the cerebellum of Lc and wt mice from P5 to adulthood. The results are expressed as the ratio of the Lc CD95 mRNA to wt CD95 mRNA, and the means ± SEM of four paired age-matched Lc and wt mice are shown. Note that CD95 levels begin to increase at the end of the episode of massive neuron degeneration (shaded in gray; see Figure 4).