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Figure 1 | Journal of Neuroinflammation

Figure 1

From: Effects of ethanol on immune response in the brain: region-specific changes in aged mice

Figure 1

Effect of ethanol on chemokine (C-C motif) ligand 2, interleukin-6, and tumor necrosis factor-α expression in aged mouse hippocampus, cerebellum, and cerebral cortex. Animals were given 6.0 g/kg/day ethanol split into two doses administered 7 hours apart. Ethanol was administered for 10 days and mice were sacrificed 24 hours after the final dose of ethanol. Water was administered to vehicle mice. Hippocampus (A), cerebellum (B), and cerebral cortex (C) were isolated, RNA prepared, cDNA synthesized and mRNA levels were determined by real-time quantitative polymerase chain reaction. Results are expressed as fold changes relative to vehicle-treated mice, and all values are normalized against β-actin. Values are mean ± standard error of the mean from 9 vehicle and 12 ethanol-treated aged mice. Duplicate polymerase chain reactions were performed on each sample. *P <0.05, **P <0.01, versus vehicle-treated mice. CCL2, chemokine (C-C motif) ligand 2; IL, interleukin; TNF, tumor necrosis factor.

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