Figure 4
Analysis of immune cell subsets in the central nervous system (CNS) post immunization with complete Freund adjuvant (CFA)/myelin oligodendrocyte glycoprotein (MOG) p35-55 . CNS mononuclear cells from unimmunized (gray bars) neonates (A, C, E) and adults (B, D, F) and from neonates and adults that had been immunized with CFA/MOGp35-55 10 days earlier (black bars). Flow cytometry was used to examine the number of CD45+ immune cells, granulocytes and microglia/g of CNS (A, B), lymphocyte subsets (C, D), and myeloid subsets in panels (E, F). Figures are representative of data obtained from at least three independent experiments.