Figure 5

3-D quantitative analyses of cerebral GFAP and COX-2 expressions. The optical staining intensities of GFAP (A) and COX-2 (D) were measured with 3-D image analysis software and shown in the cortex (CTX) and hippocampal formation (HPF) of mice maintained on low-fat control chow (LF), diet enriched in saturated fat (SFA) or each diet supplemented with nutraceutical garlic extract-aged (+GEA), alpha-lipoic acid (+ALA), niacin (+Niacin) or nicotinamide (+NA) for nine months. One-way ANOVA followed by Tukey’s post hoc test was used to analyze the statistical significance at P <0.05. Data were analyzed in the cortex and hippocampal formation separately, however they are shown as combined since the statistical significance was identical. (a) Significant difference between LF and SFA; (c) Significant difference between SFA and each nutraceutical treatment. (B and E) show the correlation between parenchymal IgG extravasation and GFAP or COX-2 expression in all groups. Pearson’s correlation coefficient is indicated. For GFAP and parenchymal abundance of plasma proteins (B and E) and for COX-2 versus IgG and apoB (C and G).