Glaucomatous neurodegeneration was more severe in D2.
mice. (A) Distributions of optic nerve damage showed a significant increase in the number of eyes with moderate (MOD; <50% axons damaged/lost) and severe (SEV; >50% axons damaged/lost) glaucoma at 10.5 months in D2.C5
B6 compared with normal D2 mice (P = 0.0001). There was no difference between D2 mice carrying one (D2.C5
D2/B6) or two functioning copies (D2.C5
B6/B6) of the C5 gene. Number of eyes: 10.5 months; D2 = 54, D2.C5
D2/B6 = 33, D2.C5
B6/B6 = 22, D2.C5
B6 (combined D2.C5
D2/B6 and D2.C5
B6/B6) = 55. 12 months; D2 = 33, B6 (combined) = 25. NOE, no or early glaucoma (no detectable axon loss but some eyes may have early molecular changes). (B) A summary of the optic nerve damage assessment shown in (A). At 10.5 months of age, 78% of eyes from D2.C5
B6 mice showed either MOD or SEV glaucoma compared with only 57% of D2 mice. C
) Examples of NOE and SEV damaged nerves in D2 and D2.C5
B6. Scale bar = 25 μm.