Significant loss of retinal ganglion cells (RGCs) in both D2 and D2.
mice. (A) Tubulin β-III (TUBB3)-labeled RGCs were counted in flat-mount retinas with no or early (NOE) or severe (SEV) glaucoma at 10.5 months of age for both DBA/2J and D2.C5
B6 mice. RGC degeneration matched the severity of optic-nerve degeneration in both groups. Eight retinas of each age/genotype were counted. (B) Example images of TUBB3-labeled RGCs in flat-mount retinas with NOE and SEV glaucomatous damage in D2 and D2.C5
B6. Scale bar = 20 μm.