Immune/inflammatory cell accumulation in brains of BALB/c, CcS-11 and STS mice after s.c. (13 day p.i.) (A) or i.c. (B) inoculation of TBEV. Immune cell accumulation in the CNS was quantified as the changes in mRNA levels of cell surface markers of B-cells (CD19), T-helper cells (CD4), cytotoxic T-cells (CD8β) and macrophage/monocyte/granulocyte/NK cells (CD11b), and normalized to expression of housekeeping gene, mouse beta actin. Data are expressed as the mean + standard deviation of the fold increase in the level of the mRNA in comparison to uninfected control mice. *p < 0.05; **p < 0.005 (n = 3 mice per group and experiment).