Figure 10

Expression and distribution of nestin (red) and GFAP (green) in the retina of sham (A-C) and BH rats (D-L). In sham retina, a weak nestin labeling is detected in GCL and IPL (A). In the GCL, weak nestin immunofluorescence is co-localized in GFAP-labeled astrocytes (single arrow, A-C). Following blast exposure, nestin expression is evidently increased in various retinal layers at different time-points (D-L) notably at 72 h and 2 weeks (G, J). Following blast exposure, nestin immunoreactivity is localized in GFAP-labeled astrocytes (single arrow) associated with blood vessels (asterisk). Nestin labeling in the vascular endothelial cells is evident (arrow head, J-L). Scale bar=20 μm (A-L).