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Figure 2 | Journal of Neuroinflammation

Figure 2

From: Primary blast injury-induced lesions in the retina of adult rats

Figure 2

Western blot analysis of VEGF, AQP4, iNOS, eNOS, nNOS, and nestin protein expression levels in the retina. Sham rats (lane 1); rats subjected to BH exposure and sacrificed at 24 h (lane 2), 72 h (lane 4), and 2 weeks (lane 6); and rats subjected to BL exposure and sacrificed at 24 h (lane 3), 72 h (lane 5), and 2 weeks (lane 7). (A) VEGF (21 kDa), AQP4 (34 kDa), iNOS (130 kDa), eNOS (140 kDa), nNOS (155 kDa), nestin (200–220 kDa), and β-actin (42 kDa) immunoreactive bands. Bar graphs representing optical density (mean±SD) of (B) VEGF, (C) AQP4, (D) iNOS, (E) eNOS, (F) nNOS, and (G) nestin normalized to β-actin for sham, 24 h, 72 h, and 2 weeks after BH and BL (* P <0.05; **P <0.01). (B) VEGF shows an acute increase at 24 h and 72 h after BH and BL exposure when compared with that of the sham rats with recovery at 2 weeks after BL exposure when compared with the sham and at 2 weeks after BH exposure. (C) AQP4 shows a steady increase at 24 h, 72 h, and 2 weeks after BH and BL exposure when compared with that of the sham rats. (D) iNOS shows a significant increase at 24 h, 72 h, and 2 weeks after BH and BL exposure when compared with that of the sham rats. (E) eNOS and (F) nNOS show increase at 24 h, 72 h, and 2 weeks after BH and BL exposure when compared with that of the sham rats with decrease at 2 weeks after BL exposure when compared with the sham and at 2 weeks after BH exposure. (G) Nestin shows an acute and significant increase at 24 h, 72 h, and 2 weeks after BH and BL exposure when compared with that of the sham rats.

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