Figure 2From: DAP12 and CD11b contribute to the microglial-induced death of dopaminergic neurons in vitro but not in vivo in the MPTP mouse model of Parkinson’s disease Microglial expression of Dap12 is induced in the mouse ventral mesencephalon following 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) intoxication. (A) Real-time quantitative PCR analysis of Dat, Cd11b and Dap12 transcripts in the ventral mesencephalon before (NaCl) and after MPTP intoxication at the indicated times. * P <0.05 compared to NaCl-treated controls; Holm-Sidak post hoc analysis. RQV, relative quantity value; d, days. (B) Double immunofluorescent staining for CD11b (red) and DAP12 (green) on mesencephalic tissue sections from control or MPTP-injected mice 2 days after toxin exposure. The arrows indicate hypertrophied and double-labeled microglial cells at the level of the SN pars compacta. Scale bar: 30 μm.Back to article page