Figure 2

Neuronal apoptosis time course in subarachnoid hemorrhage model. A. Merge of DAPI and TUNEL staining in sham and subarachnoid hemorrhage (SAH) along entire time course from post-operative days (PODs) 1 to 15. Scale Bar: 10 μm. B. Nissel stain from atlas.brain-map.org showing specific area of dentate gyrus that was used for quantification of neuronal apoptosis. C. Apoptotic neurons quantified in dentate gyrus on each POD. Student’s t-test for each POD showed significance between sham and SAH on POD 5 (P <0.02), 7 (P <0.0001), 10 (P <0.01) and 15 (P <0.0001). Error bars are standard deviations and N = 4 per group.