Immunohistochemistry of TLR4 co-localization among the different cell types of the murine brain. Representative images from four mice per group and four different fields of view on post-operative day (POD) 7 A. Immunohistochemistry across shows staining with Toll-like receptor 4 (TLR4) in the first panel, Iba1 (for microglia) in the second panel, and the merge in the third panel. Arrows delineate co-localization. B. Immunohistochemistry showing individual panels and merge for astrocytes with Glial Fibrillary acidic protein. C. Immunohistochemistry for β III Tubulin reflecting co-localization of TLR4 and neurons. Scale Bars: 15 μm. D. Quantification based on four mice and four different fields of view at POD 7 and POD 15 showing microglia express the most Toll-like receptor 4 (TLR4) by ANOVA at both time points (P <0.001).