The effects of microglial ablation on neuronal apoptosis. A. Top panel: merge of DAPI and TUNEL staining from dentate gyrus of wild type subarachnoid hemorrhage (SAH) after intracerebroventricular (ICV) PBS liposome injection on POD 7 and 15. B. Bottom panel: merge of DAPI and TUNEL staining in wild type SAH after ICV clodronate injection on POD 7 and 15. Scale Bars: 10 μm in all panels. C. Quantification of apoptotic neurons on POD 7 displaying significant reduction, by Student’s t-test (P <0.03), after ICV clodronate injection and microglial ablation. D. Quantification of apoptotic neurons on POD 15 revealing no change between ICV PBS and clodronate injections. N = 3 for each group and error bars are standard deviations.