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Figure 1 | Journal of Neuroinflammation

Figure 1

From: M-CSF increases proliferation and phagocytosis while modulating receptor and transcription factor expression in adult human microglia

Figure 1

The macrophage colony-stimulating factor (M-CSF) receptor (CSF-1R) is expressed by adult human microglia in vitro . (A) Adult human microglia in culture labeled for CD45 cell surface protein (green). (B) Same field of view showing microglial expression of PU.1 transcription factor (red). (C) Brightfield image showing 3,3′-diaminobenzidine tetrahydrochloride (DAB)-labeling of CSF-1R in the same field of view. (D) Overlay of images A, B and C demonstrating co-localization of CSF-1R with microglial markers CD45 and PU.1. (E) CSF-1R (brown) is not co-expressed by glial fibrillary acid protein (GFAP)-positive astrocytes (green) in mixed glial cultures (all nuclei labeled with Hoechst, blue). (F) CSF-1R (brown) is not expressed by prolyl-4-hydroxylase-positive fibroblast-like leptomeningeal cells (green) in mixed glial cultures (all nuclei labeled with Hoechst, blue). Scale bar = 50 μm.

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