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Figure 2 | Journal of Neuroinflammation

Figure 2

From: M-CSF increases proliferation and phagocytosis while modulating receptor and transcription factor expression in adult human microglia

Figure 2

Macrophage colony-stimulating factor (M-CSF) treatment increases the number of microglia and expression of PU.1 in primary adult human mixed glial cultures. (A) PU.1-positive microglia (red nuclei) are present in glial cultures from human brain tissue. (B) There are more PU.1-positive cells with M-CSF treatment compared to vehicle treatment. The increase in microglia number is also seen by immunostaining for microglial surface receptor CD45 (C and D). (E) Treatment of human glial cultures with 25 ng/ml M-CSF for 96 hours significantly increases the number of microglia present. P < 0.0001, n = 6. (F) M-CSF significantly increases the intensity of PU.1 expression (amount of PU.1 protein) in adult human microglia. P < 0.05, n = 6. Scale bar = 100 μm.

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Journal of Neuroinflammation

ISSN: 1742-2094