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Figure 5 | Journal of Neuroinflammation

Figure 5

From: M-CSF increases proliferation and phagocytosis while modulating receptor and transcription factor expression in adult human microglia

Figure 5

Macrophage colony-stimulating factor (M-CSF)-treated microglia assume a rod-like shape. (A and B) Immunocytochemical labeling of CD45 microglial cell surface protein shows the morphology of adult human microglia in vitro. (A) Basal adult human microglial morphology is heterogeneous - some microglia are more rounded and others are more ramified. (B) A shift in microglial shape to a rod-like morphology is evident after 48 hours M-CSF treatment. This significant elongation of microglia with M-CSF can be quantified using Metamorph Elliptical Form Factor (elongation; P < 0.001, n = 16) (C) and Shape Factor (roundness; P < 0.01, n = 16) (D) image analysis tools. Scale bar = 100 μm.

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