Figure 3
From: Diverse activation of microglia by chemokine (C-C motif) ligand 2 overexpression in brain
CCL2 overexpression induces GFP+ BMDC extravasation into mouse CNS parenchyma. Five million CD11b+ BMDCs were injected into the left ventricles of rAAV9-CCL2 mice. Photomicrographs of GFP+ infiltrating cells in the cortex (A) and (B) and hippocampus (C) and (D) are presented. Insets in (B) and (D) represent higher magnification of GFP+ cells. (E) The number of infiltrating GFP+ BMDCs was determined by cell counting in both hippocampus and cortex. CCL2 overexpression in hippocampus resulted in significantly more GFP+ cells than in the cortex in this cohort. (F) Representative data set from flow cytometry profile of one of six mice per group analyzed to detect the GFP+ cells infiltrating the brain homogenate. The percentages of cells were derived from data gated on lower forward-scatter light characteristics versus FITC fluorescence intensity. (G) Data analysis of 2,000 cells from flow cytometry where cells with fluorescence intensity greater than 1 × 104 were counted and graphed using GraphPad Prism 5 software (GraphPad Software). Student’s t-test, *P < 0.05, **P < 0.01. Scale bars represents 200 μm and 20 μm.