Figure 6

Induction of microglial markers of classical activation (M1) and alternative activation (M2) following rAAV9-CCL2 transduction. Mice which received control GFP viral vector (A), (D), (G) and (J) or mice overexpressing CCL2 (B), (C), (E), (F), (H), (I), (K) and (L) were stained using immunohistochemistry for microglial markers representing the M1 activation state: calgranulin B (Calg.B or S100-A9: (A), (B) and (C)) and IL-1β ((D), (E) and (F)); or the M2 activation state: arginase 1 (Arg-1: (G), (H) and (I)) and YM1 (J), (K) and (L). (C), (F), (I) and (L) are images of different microglial morphology stained for each marker. Scale bar is 20 μm.