Expression of MBP, CNPase and p75NTR in HSC cultures.
(A) shows HSC cultures staining with MBP (red) but not CD-90. (B) shows the expression of the myelinating marker CNPase (red) and the Schwann cell marker p75NTR (green) in HSC cultures. A representative image of HSC cultures stained with isotype control for mouse IgG1 followed by goat anti-mouse Alexa Fluor 568 is shown in (C). The nuclei of cells appear blue in A, B and C due to staining with nuclear stain TOPRO3. CNPase, 2′,3′-cyclic nucleotide 3′-phosphodiesterase; HSC, human Schwann cell; MBP, myelin basic protein.