Macrophage/microglia reactivity in EAE is reduced by dipyridamole. Iba1 staining of thoracic cord shows extensive representation of macrophage/microglia in the vehicle group (A) upon killing of the mice (at termination of the experiment in Figure 4) compared to that with dipyridamole treatment (B) (×100 original magnification). The insets in (A) and (B) are high-magnification photomicrographs (×400 original magnification) that display the amoeboid versus ramified morphology, respectively, of cells. (C) and (D) Thresholded Iba1 staining of (A) and (B), respectively, to allow the quantitation displayed in (E) and (F). The percentage of spinal cord section occupied by Iba1 staining (E) and the average macrophage/microglia area (F) are reduced by dipyridamole compared to the EAE-vehicle group. These features correlate with the clinical scores (sum of daily scores of individual mice).