Figure 2

Percentage of cell death in spinal cord injury (SCI) slices. Viable cells within the slices, identified using MTT tetrazolium dye, were visualized under light microscopy. The level of cell death was assessed in each slice at 7 days. Cell death in injured groups was significantly higher (40%) in comparison to the control group. Pretreatment with PEA and Lut alone or with the PEA + Lut association (given as combination therapy) were not able to reduce cell death. Indeed, cell death was significantly attenuated by co-ultraPEALut pretreatment at 0.0009 g/l (containing PEA and Lut at 2.7 and 0.27 μM, respectively) and 0.009 g/l (containing PEA and Lut at 27 and 2.7 μM, respectively). This figure is representative of at least three experiments performed on different experimental days. ***P <0.001 versus Ctr; ^## P <0.01 and ^### P <0.001 versus Damage; °P <0.01 versus PEA 1 μM + Lut 0.1 μM. Ctr, control; Lut, luteolin; MTT, 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; PEA, palmitoylethanolamide; SCI, spinal cord injury.