Effect of co-ultraPEALut on COX-2 expression. Homogenized spinal cord organotypic cultures were evaluated by western blot analysis, collected 24 hours after injury. (a, a1) Injury caused a significant increase in COX-2 expression compared to the control group. The pretreatment with co-ultraPEALut at 0.0009 g/l (containing PEA and Lut at 2.7 and 0.27 μM, respectively) and 0.009 g/l (containing PEA and Lut at 27 and 2.7 μM, respectively) significantly reduced COX-2 levels. Data were normalized on the basis of β-actin levels. This figure is representative of at least three experiments performed on different experimental days. **P <0.01 versus Ctr; ##
P <0.01 versus Damage. Ctr, control; COX-2, cyclooxygenase-2; PEA, palmitoylethanolamide; Lut, luteolin.