Figure 5

CXCR4 expression is increased on oligodendrocytes in IκBα-dn mice. (A) Western blot analysis of naïve and injured spinal cords showed a significant increase in CXCR4 protein levels in IκBα-dn mice compared to wild-type (WT) mice 6 weeks post-spinal cord injury (SCI). (B) Representative photomicrographs of mature CC1⁺CXCR4⁺ oligodendrocytes in IκBα-dn and WT spinal cord 6 weeks after SCI. (C) Representative photomicrograph of a NG2⁺CXCR4⁺ in WT and IκBα-dn spinal cord 6 weeks post-SCI. (D) Western blot analysis for Foxc2 shows significantly increased expression in IκBα-dn mice compared to WT mice 6 weeks post-SCI. (E) CXCR7 expression following SCI, examined by Western blot analysis, showed that CXCR7 levels were significantly increased only in WT mice 6 weeks after SCI, whereas no significant increase was observed in IκBα-dn mice. Each bar represents mean ± SEM. N = 3 to 4 animals per group. *P < 0.05 and **P < 0.01. NG2, nerve/glial antigen 2.