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Figure 4 | Journal of Neuroinflammation

Figure 4

From: T-cell reconstitution during murine acquired immunodeficiency syndrome (MAIDS) produces neuroinflammation and mortality in animals harboring opportunistic viral brain infection

Figure 4

Dual infection triggered brain infiltration by peripheral immune cells and corresponding microglial cell activation. Single cell suspensions of brain tissue obtained from LP-BM5-, herpes simplex virus (HSV)- and LP-BM5 + HSV-infected mice (three to five animals per time point) were banded on a 70% Percoll cushion. Brain leukocytes were collected and labeled with PE-Cy5-conjugated Abs specific for CD45, AF700-labeled anti-CD11b, and allophycocyanin (APC)-labeled major histocompatability complex (MHC) class II. Analysis was done by using flow cytometry (FacsCanto, BD Biosciences, CA), with FlowJo software (TreeStar, Inc.). (A) The dot plots presented are representative of three experiments at 7 and 14 d post-infection (p.i.). The CD45hi population, representing peripheral immune cells, are clearly differentiated from the CD45int resident microglial cells. (B) The percentages of CD45hi cells among each group pooled from three independent flow cytometry experiments at 7 and 14 d p.i. with HSV are shown. (C) CD45intCD11b+ microglia were analyzed for upregulation of the activation marker MHC class II. Representative histograms from three independent experiments are shown. (D) The percentages of MHC class II expression on microglial cells at 14 d p.i. are shown. * P <0.01 versus HSV infection alone.

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