Figure 5

Allergy decreases the levels of insulin-degrading enzyme (IDE) in the mouse brain. Western blot analysis showed a decrease in IDE levels in the hippocampus (A), frontal cortex (B) and hypothalamus of allergic mice compared to controls (C). The data were analyzed using Student’s t-test. The boxplot indicates the first two quartiles with the median, and the whiskers indicate the third and fourth quartiles (n = 8 mice per group). Only six control mice were used for the hypothalamus because two samples were lost during sonication. The images below the graph indicate the immunoblotting of IDE. Asterisk, p < 0.05, triple asterisk p < 0.001.