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Table 2 Assay performance

From: The effect of intravenous interleukin-1 receptor antagonist on inflammatory mediators in cerebrospinal fluid after subarachnoid haemorrhage: a phase II randomised controlled trial

Analyte Plasma CSF
  Sensitivity (pg/mL) Inter-assay CV range (QC concentration range) Sensitivity (pg/mL) Inter-assay CV range (QC concentration range)
IL-1Ra 16 8.2 to 3.2 (360 to 10,009 pg/mL) Same assay as for plasma
IL-1α 6.1 9.0 to 4.2 (25 to 366 pg/mL) 4.75 6.6 to 9.2 (20 to 800 pg/mL)
IL-1β 1.3 10.3 to 6.3 (17.5 to 146 pg/mL) 0.48 30.3 to 10.9 (1.3 to 10.9 pg/mL)
IL-6 1.7 5.9 to 2 (41 to 322 pg/mL) 17 7.1 to 2.6 (213 to 1,229 pg/mL)
IL-8 4.7 7.9 to 16 (109 to 975 pg/mL) 17 8.9 to 15.8 (185 to 9,152 pg/mL)
IL-10 1.3 10.3 to 6.26 (7.5 to 146 pg/mL) 0.82 10.6 to 5.5 (5.16 to 232 pg/mL)
MCP-1 45 19.5 to 11.3 (207 to 1,759 pg/mL) 147 (19 to 6.5 (9,303 to 11,275 pg/mL)
TNF-α 0.35 6.7 to 7.0 (16.8 to 159 pg/mL) 2.5 25 to 15.8 (4.3 to 28.1 pg/mL)
CRP 350 14.8 to 1 (9.2 to 97 mg/L) ND
  1. The assay performance data is only for assays used in this study. The lowest sensitivity of the assays is shown and represents either the non-sample value (+2 SD), or the lowest point on the standard curve. Inter-assay CVs are shown from the lowest to the highest concentration of QCs (three or four) that were included in all assays. CRP, C-reactive protein; CV, coefficient of variation; IL, interleukin; IL-1Ra, interleukin-1 receptor antagonist; MCP-1, monocyte chemoattractant protein-1; ND, not determined; QC, quality control; TNF, tumour necrosis factor.