Astro KO and Endo KO mice show different patterns of clinical EAE. EAE was induced in WT, Astro KO, and Endo KO mice by MOG35-55 immunization; all mice were observed daily and scored for clinical disease for 30 days. Each group consisted of 5–6 mice, and analysis was performed in triplicate. Graphs represent mean data points from all three analyses ± standard error. (a) Mean clinical EAE scores. Astro KO mice do not attain as severe disease as WT during the evaluation period, while Endo KO mice approach WT disease severity but do so only after significantly delayed onset. The rate of rise of clinical disease, as reflected by the slope of each regression line (hatched lines) through the respective ascending disease scores for the different mice, is similar in both WT and Endo KO mice, but notably less in Astro KO mice. (b) Disease incidence. All mice show a similar incidence of disease but, compared to WT mice, Astro KO mice show only a mild delay while Endo KO mice show a prolonged delay in disease onset. (c) Summary of various clinical disease parameters among the three mouse groups.