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Table 1 Number of rats killed at various time points in different groups

From: Hypertonic saline alleviates cerebral edema by inhibiting microglia-derived TNF-α and IL-1β-induced Na-K-Cl Cotransporter up-regulation

 

BWC measurement

TTC staining

Double immunofluorescence

Real time RT-PCR

Western blotting

Sham-operated group

     

12 h

7

0

10

6

6

24 h

7

6

10

0

0

Cerebral ischemic group

     

12 h

7

0

10

6

6

24 h

7

6

10

0

0

Cerebral ischemic + 10% HS group

     

12 h

7

0

10

6

6

24 h

7

6

10

0

0

  1. BWC, brain water content; TTC, 2,3,5-triphenyltetrazolium chloride.