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Table 2 Antibodies used in experiments

From: Hypertonic saline alleviates cerebral edema by inhibiting microglia-derived TNF-α and IL-1β-induced Na-K-Cl Cotransporter up-regulation

Antibody

Host

Company

Cat. No.

Application

Concentration

GFAP

Mouse

Millipore, Billerica, MA, USA

MAB360

Tissue/cell (IHC, IF, WB)

1:100

GFAP

Mouse

Santa Cruz Biotechnology, Santa Cruz, CA, USA

sc-58766

Cell (IF)

1:200

OX42

Mouse

Millipore, Billerica, MA, USA

CBL1512Z

Tissue (IHC)

1:50

Iba-1

Mouse

abcam, Cambridge, MA, USA

ab15690

Cell (IF)

1:200

NKCC1

Rabbit

Millipore, Billerica, MA, USA

AB 3560P

Tissue/cell (IHC, IF, WB)

1:100

NKCC1

Goat

Santa Cruz Biotechnology, Santa Cruz, CA, USA

sc-21545

Cell (IF, WB)

1:50

TNF-α

Rabbit

Millipore, Billerica, MA, USA

AB1837P

Tissue (IHC, WB)

1:50

TNF-α

Rabbit

Chemicon International, Temecula, CA, USA

AB1837P

Cell (IF)

1:400

IL-1β

Rabbit

Abcam, Cambridge, MA, USA

ab9787

Tissue (IHC, WB)

1:100

IL-1β

Rabbit

Chemicon International, Temecula, CA, USA

AB1832P

Cell (IF)

1:400

Phos-p38

Rabbit

Cell Signaling Technology, Danvers, MA, USA

4511S

Cell (WB)

1:1,000

P38

Rabbit

Cell Signaling Technology, Danvers, MA, USA

8690S

Cell (WB)

1:1,000

Phos-JNK

Rabbit

Cell Signaling Technology, Danvers, MA, USA

4668S

Cell (WB)

1:1,000

JNK

Rabbit

Cell Signaling Technology, Danvers, MA, USA

9252S

Cell (WB)

1:1,000

β-actin

Rabbit

Cell Signaling Technology, Danvers, MA, USA

4970

Cell (WB)

1:1,000

GAPDH

Goat

Santa Cruz Biotechnology, Santa Cruz, CA, USA

sc-20357

Tissue (WB)

1:1,000

  1. IF, immunofluorescent; IHC, Immunohistochemistry; WB, Western blotting.