Figure 10

MCP-1 is induced in astrocytes in response to pro-inflammatory cues. Representative images of cells in mixed glial cultures treated for 24 hours with vehicle (0.1% BSA in PBS), TNFα (50 ng/ml), IL-1β (10 ng/ml) or LPS (10 ng/ml) +/- IFNγ (10 ng/ml) labelled with MCP-1 (green), GFAP (red) and Hoechst (blue), scale bar = 100 μm. GFAP, glial fibrillary acidic protein; LPS, lipopolysaccharide; MCP-1, monocyte chemotactic protein-1.