Figure 13From: A role for human brain pericytes in neuroinflammation IP-10 and MCP-1 are expressed and secreted by primary human brain pericytes. A) Western blot analysis of media and lysates from brain pericytes treated with vehicle (0.1% BSA in PBS), TNFα (50 ng/ml), IL-1β (10 ng/ml) or LPS (10 ng/ml) +/−IFNγ (10 ng/ml) for 24 hours. Images are representative of experiments repeated in three separate cases. B) Immunocytochemical analysis of IP-10 and MCP-1 (both green) induction by IFNγ and IL-1β (both 10 ng/ml) treatment in αSMA (red) positive pericytes with Hoechst (blue) overlay. Scale bar = 100 μm. Images are representative of experiments repeated in three separate cases. IP-10, interferon inducible protein-10; LPS, lipopolysaccharide; MCP-1, monocyte chemotactic protein-1; αSMA, alpha smooth muscle actin.Back to article page