Figure 14

Time-dependent expression of IP-10 and MCP-1 in response to pro-inflammatory cytokines in adult human brain pericytes. A) Pericyte cultures treated for 0 to 96 hours with TNFα (50 ng/ml), IL-1β (10 ng/ml) or LPS (10 ng/ml) +/−IFNγ (10 ng/ml), were stained by immunocytochemistry for IP-10, and Hoechst and quantified by Hoechst positive cells from triplicate wells, four images per well. *indicates significance (P <0.01), ***indicates significance (P <0.0001). Data are from one representative experiment that was repeated in two separate cases. B) Pericyte cultures treated as in (A) were stained by immunocytochemistry for MCP-1 and quantified as above. IP-10, interferon inducible protein-10; LPS, lipopolysaccharide; MCP-1, monocyte chemotactic protein-1.