Figure 3

Mixed glial cultures express chemokines IP-10 and MCP-1 when stimulated with pro-inflammatory molecules. Quantification of cells immunocytochemically positive for IP-10 or MCP-1 in mixed glial cultures after 24 hours treatment with vehicle (0.1% BSA in PBS), interferon γ (IFNγ), tumor necrosis factor α (TNFα), interleukin 1β (IL-1β) or lipopolysaccharide (LPS). Values plotted are percentages of total cells as measured by Hoechst positive cells from triplicate wells, four images per well. Data are representative of results from three separate cases. *indicates significance (P <0.01), ***indicates significance (P <0.0001). IP-10, interferon inducible protein-10; MCP-1, monocyte chemotactic protein-1.