Figure 4

IP-10 expression is synergistically increased in response to combination IFNγ and TNF α/IL-1β in human mixed glial cultures. A). Quantification of IP-10 positive cells in mixed glial cultures treated for 24 hours with vehicle (0.1% BSA in PBS), TNFα (50 ng/ml), IL-1β (10 ng/ml) or LPS (10 ng/ml) +/−IFNγ (10 ng/ml), from triplicate wells, at least four images per well. *indicates significance (P <0.01), ***indicates significance (P <0.0001). Data are from one representative experiment that was repeated in three separate cases. B) Quantification of the integrated intensity of IP-10 staining of cells treated in (A), ***indicates significance (P <0.0001). C) Quantification of the percentage of αSMA, CD45 and GFAP positive cells that express IP-10 under conditions in (A), *indicates significance (P <0.01), **(P <0.001), ***(P <0.0001). GFAP, glial fibrillary acidic protein; IP-10, interferon inducible protein-10; LPS, lipopolysaccharide; αSMA, alpha smooth muscle actin.