Table 2 Gene expression changes in microarray and qRT-PCR validation
Genes upregulated | Gene names | Fold change microarray | Fold change qRT-PCR |
|---|---|---|---|
HLA-DMA | major histocompatibility complex, class II, DM alpha | 3.70 +/- 0.33 | 9.86 +/- 3.72 |
ATF3 | activating transcription factor 3 | 2.33 +/- 1.0 | 5.49 +/- 0.45 |
ICAM1 | intercellular adhesion molecule 1 | 6.13 +/- 0.56 | 80.80 +/- 23.88 |
CXCL11 | chemokine (C-X-C motif) ligand 11 | 9.40 +/- 0.65 | 246926 +/- 216491 |
IL-6 | interleukin-6 | 5.82 | 117.69 +/- 17.29 |
IL-8 | interleukin-8 | 7.5 +/- 0.63 | 193.19 +/- 62.31 |
CD74 | CD74 molecule, major histocompatibility complex, class II invariant chain | 3.47 +/- 1.22 | 160.96 +/- 142.58 |
SOD2 | superoxide dismutase 2, mitochondrial | 4.35 +/- 1.03 | 27.38 +/- 3.90 |
APOL1 | apolipoprotein L, 1 | 4.19 +/- 0.27 | 25.56 +/- 7.87 |
APOL2 | apolipoprotein L, 2 | 3.46 | 8.34 +/- 1.94 |
RIPK2 | receptor-interacting serine-threonine kinase 2 | 2.04 +/- 0.25 | 3.16 +/- 0.24 |
IRF1 | interferon regulatory factor 1 | 4.48 +/- 0.39 | 21.55 +/- 3.25 |
PTGS2 | prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) | 3.28 +/- 0.82 | 8.49 +/- 1.09 |
Genes downregulated | Â | Â | Â |
CREB5 | cAMP responsive element binding protein 5 | -1.80 +/- 0.83 | 0.1 +/- 0.03 |
TNFRSF10D | tumor necrosis factor receptor superfamily, member 10d, decoy with truncated death domain | -4.78 | 0.05 +/- 0.02 |
CXCL12 | chemokine (C-X-C motif) ligand 12 | -1.79 +/- 0.25 | 0.39 +/- 0.18 |