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Figure 10 | Journal of Neuroinflammation

Figure 10

From: Early release of high-mobility group box 1 (HMGB1) from neurons in experimental subarachnoid hemorrhage in vivo and in vitro

Figure 10

Representative photomicrographs showed brain neurons double immunofluorescent staining for High-mobility group box 1 (HMGB1) (red) and Neuron-specific nuclear protein (NeuN), a neuronal cell marker (green) in vitro in the control (C, E) and Hb incubation group (D, F). (A) Immunofluorescence staining shows more than 98% cells were positive for both Neuron and 4',6-diamidino-2-phenylindole (DAPI), which suggests the high percentage of Neuron cells in the primary cultured cells. (B) Result of western blot analysis of concentrated conditioned culture media, which showed that HMGB1 could be detected in the media. (C, D) Images of cultured neurons in light microscopy to watch its sharp; light micrograph of neurons shows cellular morphology in sham group (C) and Hb incubation group (D). (E, F) Merged images of HMGB1(red) and DAPI (blue) in cultured neurons. Compared with the sham group (E), the Hb incubation groups (F) showed translocation of HMGB1 in cytoplasm (as shown by white arrows). Scale bar: 20 μm.

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