Figure 11

Conditioned medium from Hb-treated neurons induced IL-1β in cultured mixed glial cells, which could be inhibited by High-mobility group box 1 (HMGB1)-specific inhibitor. Cultured mixed glial cells were arranged into three groups. In the control group mixed glial cells were treated with control medium; in the medium group mixed glial cells were treated with neuron medium; in the medium + glycyrrhizic acid (GA) group, after mixed glial cells were treated with neuron medium, a special inhibitor of HMGB1 (GA) was added in the medium to silence the activity of HMGB1. IL-1β was measured by real-time PCR. Bars represent the mean ± standard error (n = 6), *P < 0.05 compared with the medium group; ^# P < 0.01 compared with the control group.