Figure 3

Cytosolic expression of high-mobility group box 1 (HMGB1) in Neuron-specific nuclear protein(NeuN)-positive cells of cortex from animals with subarachnoid hemorrhage (SAH). (A, B, C) HMGB1 immunostaining images obtained from the cortex of a sham (A), a 2-h SAH (B), and a day-1 (C) animal respectively. (D, E, F) Merged images of HMGB1 immunostaining (red) and 4',6-diamidino-2-phenylindole(DAPI) nuclear staining (blue). (G, H, I) Merged images of HMGB1 immunostaining (red) and NeuN immunostaining (green). Enlarged images on the right side of each panel highlight the increased number of NeuN-positive cells with cytosolic staining of HMGB1 in cortex from SAH animals. Arrows indicate the cytosolic HMGB1. The mark (>) indicates co-localization of cytosolic HMGB1 and NeuN, The results indicate that HMGB1 translocation occurred as early as 2 h and advanced in the process after SAH. Scale bars represent 20 μm.