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Figure 5 | Journal of Neuroinflammation

Figure 5

From: Early release of high-mobility group box 1 (HMGB1) from neurons in experimental subarachnoid hemorrhage in vivo and in vitro

Figure 5

Cytosolic expression of High-mobility group box 1 (HMGB1) in ionized calcium binding adaptor molecule 1 (Iba1)-positive cells of cortex from animals with subarachnoid hemorrhage (SAH). (A, B) HMGB1 immunostaining images obtained from cortex of a sham (A) and a day-1 SAH (B) animal. (C, D) Merged images of HMGB1 immunostaining (red) and 4',6-diamidino-2-phenylindole (DAPI) nuclear staining (blue). (E, F) Merged images of HMGB1 immunostaining (red) and Iba1 immunostaining (green). Enlarged images (a, b , c , d , e , f) on the right side of each panel highlight the increased number of cytosolic HMGB1-positive cells (arrows) in cortex from SAH animals and some cytosolic HMGB1-positive cells were also positive for Iba1(shown as >). Scale bars represent 20 μm. Arrows indicat the cytosolic HMGB1. The mark (>) indicates co-localization of cytosolic HMGB1 and Iba-1. The results indicate that microglia were also a source of extracellular HMGB1. Scale bars represent 20 μm.

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