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Figure 6 | Journal of Neuroinflammation

Figure 6

From: Early release of high-mobility group box 1 (HMGB1) from neurons in experimental subarachnoid hemorrhage in vivo and in vitro

Figure 6

Expression of High-mobility group box 1 (HMGB1) protein and mRNA level in brain cortex after subarachnoid hemorrhage (SAH). (A) Western blot analysis of HMGB1 expression in the total protein extraction of the cortex after SAH showed that the HMGB1 protein level was significantly increased as early as 2 h and peaked approximately on day 1. (B) Western blot analysis of HMGB1 in the cytosolic protein fraction of the cortex. The quantity of HMGB1 protein in cytosolic protein fraction was also detected to increase in the 2-h, 12-h, day-1 and day-2 groups, which indicated that the HMGB1 translocation occurred as early as 2 h after SAH onset. (C) Real-time PCR analysis of HMGB1 mRNA level demonstrated the HMGB1 mRNA level was also up-regulated as early as 2 h after SAH. Bars represent the mean ± standard error (n = 6, each group). # P < 0.01 compared with the sham group; *P < 0.05 compared with sham group.

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