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Figure 7 | Journal of Neuroinflammation

Figure 7

From: Early release of high-mobility group box 1 (HMGB1) from neurons in experimental subarachnoid hemorrhage in vivo and in vitro

Figure 7

Representative photomicrographs show brain cortex double-staining of immunofluorescent (High-mobility group box 1 (HMGB1), green) and PI staining (red) in the sham (A - E), 2-h (F - J), day -1 (K - O), and another day-1 (P - T) group. The nucleus was counterstained with 4',6-diamidino-2-phenylindole (DAPI) (blue) in the same view in each section. (D, I, N, S) Merged images of HMGB1(green) and DAPI (blue); (E, J, O, T) merged images of propium iodide (PI) (red) and HMGB1 (green). Compared with the sham group (D), the subarachnoid hemorrhage (SAH) groups (I, N, S) show translocation of HMGB1 from nuclear to cytoplasm (the mark (>) indicates the cytosolic HMGB1). Few PI-positive cells could be observed in the sham group (B, E) while patches of cells positive for PI could be detected in the SAH groups (G, L). Overlapping images (J, O) showed that most cells were positive for both cytosolic HMGB1 and PI staining (arrows indicate the co-localization of cytosolic HMGB1 and PI). However, positive staining for cytosolic HMGB1 but not positive for PI staining could also easily found in the day-1 group post SAH (T). Scale bar: 20 μm. These results support the theory that both passive and active release of HMGB1 are involved in the translocation process.

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