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Figure 9 | Journal of Neuroinflammation

Figure 9

From: Early release of high-mobility group box 1 (HMGB1) from neurons in experimental subarachnoid hemorrhage in vivo and in vitro

Figure 9

Representative photomicrographs showed brain neurons double immunofluorescent staining for cleaved caspase 3 (red) and Neuron-specific nuclear protein (NeuN), a neuron cell marker (green) in vivo in the control (A to D) and recombinant High-mobility group box 1 (rHMGB1) treatment group (E to H). The nucleus was counterstained with 4',6-diamidino-2-phenylindole (DAPI) (blue) in the same view in each section. (D, H) Merged images of cleaved caspase 3 (red) and NeuN(green). Compared with the control group (A), more cleaved caspase 3-positive cells were detected in the cortex after rHMGB1 treatment (E). Especially, overlapping images (H) showed that the number of cells positive both for cleaved caspase 3 and NeuN increased compared with control group (D). The marks (>): profiles positive for cleaved caspase 3 and DAPI but negative for NeuN showed activation of caspase 3 in non-neuronal cells. Arrows: profiles positive for cleaved caspase 3 and NeuN showed activation of caspase 3 in neurons. Scale bar: 20 μm. These results indicate that rHMGB1 addition increased the cleaved-caspase 3 positive cells, especially the neurons. RHMGB1 might promote the cell apoptosis.

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