Adjudin reduced ischemia/reperfusion induced cytokine production. Bar graph shows the mRNA levels of TNF-α (A), IL-1β (B) and IL-6 (C) in brain tissue 3 d after reperfusion. Data were expressed as average copies per copy of Rplp0, and normalized to sham group, from five separate experiments. Relative protein levels of TNF-α (D), IL-1β (E) and IL-6 (F) in blood samples were measured with ELISA. Data were normalized against the protein level of the sham group. Data were presented as mean ± SEM, n = 5 per group. *P <0.05, **P <0.01, ***P <0.005.